STUDIES ON INTERFERON PRODUCTION AND ITS INHIBITION IN CHICK EMBRYO CELLS

Interferon production in chick-embryo cells. The effect of puromycin and p-fluorophenylalanine.

1. When chick-embryo cells were treated with ultraviolet-inactivated influenza virus (Melbourne strain), interferon was produced after a lag period of about 10hr. 2. The addition of small amounts of either puromycin or p-fluorophenylalanine immediately after the virus inhibited the subsequent production of interferon. Both inhibitors primarily affected protein synthesis, and it is concluded tha...

Interferon production and viral ribonucleic acid synthesis in chick embryo cells.

Inhibition studies on tissue cultures of Walker carcinoma 256 and chick embryo heart cells.

Chick embryo heart cells were used in coverslip cultures. The medium consisted of: Chick embryo extract 15% Phenol red solution (.04%) 7% Tyrode solution 28% Chicken plasma 50% Cultures were incubated for 72 hours at 37°C. The amount of inhibition of growth was determined from comparisons of the areas of growth and of the mitotic indexes. Many of the compounds were synthesized in this Laborato...

Interferon production and protein synthesis in chick cells.

Friedman, Robert M. (National Cancer Institute, Bethesda, Md.). Interferon production and protein synthesis in chick cells. J. Bacteriol. 91:1224-1229. 1966.-Overnight incubation of chick embryo fibroblasts (CEF) at 4 C before infection with live Semliki Forest virus (SFV) increased virus yields but decreased interferon production. The same findings were noted when CEF were incubated for 4 hr w...

Interferon action II. Membrane-bound alkaline ribonuclease activity in chick embryo cells manifesting interferon-mediated interference.

Membrane fractions from chick embryo cells manifesting viral interference mediated by interferon or poly(I)-poly(C) contain high levels of an alkaline ribonuclease. Enhanced RNase activity is not observed when inhibitors of cell protein or RNA synthesis are present during interferon treatment, or when heterologous interferon is used. The RNase associated with comparable membrane fractions from ...